RealFeedApp Klf9 and Klf13 repress axon growth in the mouse brain
A multiplexed CRISPRi knockdown strategy revealed a shift from Klf6/Klf7 activators to Klf9/Klf13 repressors regulating postnatal neuronal maturation in mice.
- Field
- the brain and neuroscience
- What they did
- Researchers investigated how KLF transcription factors switch from activators to repressors during the maturation of the mouse neocortex. They used a multiplexed CRISPRi knockdown strategy to analyze chromatin accessibility and gene expression in neonatal versus maturing neurons.
- Why it matters
- This helps understand the genetic programs controlling postnatal neuronal maturation, as the mechanisms regulating this phase were previously comparatively lacking.
#crispri#neocortex#klf#transcription factors#brain development
