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- Digital PCR counts molecules without relying on a standard curve
Countable Labs partitions samples into droplets, but exceeding concentration thresholds forces the system to rely on Poisson statistics instead of direct counting.
- Field
- genetics and genomes
- What they did
- Researchers tested digital PCR technology that counts individual DNA molecules, but which becomes inaccurate when multiple molecules enter a single compartment.
- Why it matters
- This is crucial for accurately quantifying nucleic acids in samples with high concentrations, as standard methods fail to provide reliable results in such conditions.
#digital pcr#single molecule counting#countable labs#persistence#multiplexing
